phenol red (Thermo Fisher)

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Thermo Fisher phenol red
Phenol Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple/TrypLE+Select+Enzyme+(1X)/pm41665641-208-5-7
Average 96 stars, based on 1 article reviews

phenol red - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Disruption of Cell-Adhesion Signaling Resolves Unwanted Progenitor Specification in Stem Cell-Derived α and β Cell Grafts.
Article Snippet: .. Clusters of differentiated cells were dissociated using TrypLE (Thermo Fisher, Waltham, MA, USA) at 37 ◦C and fixed in 4% PFA for 1 h. Clusters were blocked using PBS + 0.1% Triton X-100 + 5% donkey serum for 1 h. Cells were incubated in primary antibodies for 1 h at room temperature. ..

Article Title: O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cells
Article Snippet: .. Following crypt isolation, crypts were resuspended in TrypLE (Gibco TM , no phenol red, 12604039) and dissociated into single cells through a 60-second incubation at 32°C. .. Dissociated single cells were washed with 10ml of S-MEM (Gibco TM , 11380037) and pelleted (300 g, 5 min, 4°C).

Article Title: Hypoxia-inducible factor 1 protects neurons from Sarm1-mediated neurodegeneration.
Article Snippet: Dissociation DRGs from up to 8 E13 or E14 mouse embryos were collected in a sterile 2 mL tube in L-15 media (Thermo Scientific 11415064) and washed three times in L-15 (centrifugation at 200g for 30 s). .. After removing the supernatant completely, TrypLE (Thermo 12605010; containing recombinant trypsin and EDTA) was added (1 mL for DRGs from up to 8 embryos), and the tube was immediately submerged in a 37 ◦ C water bath and incubated for 5–6 min (during incubation, the tube was briefly inverted 2 or 3 times; keeping digestion time below 6 min was found to be essential). ..

Isolation:

Article Title: Maternal obesity induces developmental programming of Intestinal stem cells through an IL-17A/PPAR immune-epithelial axis
Article Snippet: .. For sorted ISC cultures, following crypt isolation, crypts were resuspended in TrypLE (GIBCO, no phenol red, 12604039) and dissociated into individual cells by heat shocking at 32°C for 90 seconds. .. Dissociated single cells were stained with the following antibody cocktail for flow cytometry analysis: CD44 (Biolegend, 103041), EpCAM-APC (eBioscience, 17-5791-82), DAPI (BioLegend, 422801).

Recombinant:

other:

Article Title: Impact of cell culture transition on adipose-derived mesenchymal stromal cells: Multi-omics analysis of small- vs large-scale production.
Article Snippet: After 14 days in culture, the cells were harvested using a standard trypsinization protocol (TrypLE; Gibco, Thermo Fisher Scientific).

Suspension:

Article Title: DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells
Article Snippet: .. Cells were treated with Trypsin (Quality Biological) or TrypLE (ThermoFisher Scientific) (4 mL Trypsin/TrypLE per 15 cm plate of adherent cells, 1 mL Trypsin/TrypLE per 1 million suspension cells) for 10 minutes at 37°C and the resulting Trypsin-cell mixture was spun down twice at 400xg for 3 minutes at room temperature to pellet and remove cells. .. Trypsin was lyophilized and resuspended in 1 mL nuclease-free water, followed by the addition of 2 mL (2 volumes) DNA binding buffer (Zymo Research) and 6 mL (2 volumes water + DNA binding buffer) 100% ethanol, vortexing between each addition.

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