tryple express enzyme (Thermo Fisher)

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Thermo Fisher tryple express enzyme
Tryple Express Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple/TrypLE+Express+Enzyme+(1X)/pmc13651307-106-8-14
Average 99 stars, based on 1 article reviews

tryple express enzyme - by Bioz Stars, 2026-11

99/100 stars

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Incubation:

Article Title: Disruption of Cell-Adhesion Signaling Resolves Unwanted Progenitor Specification in Stem Cell-Derived α and β Cell Grafts.
Article Snippet: .. Clusters of differentiated cells were dissociated using TrypLE (Thermo Fisher, Waltham, MA, USA) at 37 ◦C and fixed in 4% PFA for 1 h. Clusters were blocked using PBS + 0.1% Triton X-100 + 5% donkey serum for 1 h. Cells were incubated in primary antibodies for 1 h at room temperature. ..

Article Title: O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cells
Article Snippet: .. Following crypt isolation, crypts were resuspended in TrypLE (Gibco TM , no phenol red, 12604039) and dissociated into single cells through a 60-second incubation at 32°C. .. Dissociated single cells were washed with 10ml of S-MEM (Gibco TM , 11380037) and pelleted (300 g, 5 min, 4°C).

Article Title: Hypoxia-inducible factor 1 protects neurons from Sarm1-mediated neurodegeneration.
Article Snippet: Dissociation DRGs from up to 8 E13 or E14 mouse embryos were collected in a sterile 2 mL tube in L-15 media (Thermo Scientific 11415064) and washed three times in L-15 (centrifugation at 200g for 30 s). .. After removing the supernatant completely, TrypLE (Thermo 12605010; containing recombinant trypsin and EDTA) was added (1 mL for DRGs from up to 8 embryos), and the tube was immediately submerged in a 37 ◦ C water bath and incubated for 5–6 min (during incubation, the tube was briefly inverted 2 or 3 times; keeping digestion time below 6 min was found to be essential). ..

Isolation:

Article Title: Maternal obesity induces developmental programming of Intestinal stem cells through an IL-17A/PPAR immune-epithelial axis
Article Snippet: For crypt cultures, isolated crypts were counted and embedded in 3.5:6.5 media: Matrigel growth factor reduced (Corning, 356231) mixture at 5-10 crypts per μL and cultured in a crypt culture medium modified from Sato et al. (2009). .. For sorted ISC cultures, following crypt isolation, crypts were resuspended in TrypLE (GIBCO, no phenol red, 12604039) and dissociated into individual cells by heat shocking at 32°C for 90 seconds. .. Dissociated single cells were stained with the following antibody cocktail for flow cytometry analysis: CD44 (Biolegend, 103041), EpCAM-APC (eBioscience, 17-5791-82), DAPI (BioLegend, 422801).

Recombinant:

other:

Article Title: Impact of cell culture transition on adipose-derived mesenchymal stromal cells: Multi-omics analysis of small- vs large-scale production.
Article Snippet: After 14 days in culture, the cells were harvested using a standard trypsinization protocol (TrypLE; Gibco, Thermo Fisher Scientific).

Suspension:

Article Title: DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells
Article Snippet: .. Cells were treated with Trypsin (Quality Biological) or TrypLE (ThermoFisher Scientific) (4 mL Trypsin/TrypLE per 15 cm plate of adherent cells, 1 mL Trypsin/TrypLE per 1 million suspension cells) for 10 minutes at 37°C and the resulting Trypsin-cell mixture was spun down twice at 400xg for 3 minutes at room temperature to pellet and remove cells. .. Trypsin was lyophilized and resuspended in 1 mL nuclease-free water, followed by the addition of 2 mL (2 volumes) DNA binding buffer (Zymo Research) and 6 mL (2 volumes water + DNA binding buffer) 100% ethanol, vortexing between each addition.

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